Micropipette tips are disposable, specially designed attachments used with micropipettes to accurately transfer small volumes of liquids in laboratory settings. These tips ensure precise measurement and prevent contamination during experiments. They come in various sizes and are typically made of plastic to handle different volumes of liquid.

 

What are Micropipette tips?

A micropipette is a vital scientific tool used globally in laboratories for the accurate transfer of measured liquid volumes between containers. Whether a simple plastic tube or a high-precision electronic device, all pipettes rely on one essential component: the micropipette tip. This disposable plastic tip is critical to the instrument's functionality. However, choosing the right tip is not as straightforward as it seems. Factors such as ergonomics, precision, accuracy, dimensions, and quality must be considered, as even a slight variation can affect the pipette’s performance and the experiment's results. Micropipette with tips are widely used in fields like life sciences, chemistry, and molecular biology, and must meet stringent quality standards such as ISO 8655. In biological research, tips often include filters to prevent contamination and ensure reliable, accurate results.

 

Types and Variations in Micropipette tips:

Filter vs Non-Filter Tips:

Filter Tips: Filter tips are ideal for handling corrosive, volatile, or viscous samples that could damage the pipette. They feature a filter that prevents over-aspiration, protecting the pipette shaft from contamination. These tips are also useful for novice technicians or students in training, offering cost-effective protection against sample contamination or damage to equipment.

Non-Filter Tips: These are the standard, cost-effective options used in most labs. Non-filter tips are better suited for experienced users handling non-sensitive samples, as they lack the contamination-prevention features of filter tips. They are typically used for applications like DNA isolation and loading gels, where contamination risk is low.

 

By Packaging:

Racked: Micropipette with tips come pre-filled in sturdy, stackable racks made of polypropylene. These racks, with transparent lids and bases, are reusable once emptied and offer convenient, contamination-free handling.

Bulk: Bulk tip micropipette  are packed in resealable zip-lock polyethylene bags, providing good protection against contamination. Users must manually transfer the tip micropipette  to sterile boxes.

Reloads: Reload packs are more sustainable and environment-friendly, allowing the reuse of the same rack with replacement reloads. These come with a nestable, compact design for contamination-free usage.

Individually Wrapped: These tips are packaged singly to ensure the highest sterility standards, making them ideal for highly sensitive applications that require stringent aseptic conditions.

 

Low Retention vs Non-Low Retention Tips:

Low Retention Tips: These tips are designed to prevent liquid from sticking to their surfaces. Manufactured through techniques like mechanical polishing or incorporating hydrophobic materials, they ensure precise pipetting by reducing sample loss. However, chemical coatings may risk leaching, causing contamination or uneven pipetting results.

Non-Low Retention Tips: Standard tips without surface treatments, used when sample retention is not a concern.

 

Benefits of Using Micropipette tips:

Liquid handling is a critical aspect of laboratory experiments, especially when working with very small volumes in the microliter range. In such cases, pipette tips are essential tools that simplify the process. Here’s how pipette tips improve liquid sample handling:

• Deliver high accuracy and precision in measurements
• Simple and user-friendly design
• Available in sterile options for contamination-free experiments
• Efficient, saving both time and effort in liquid transfer

 

Uses and Applications of Micropipette tips:

Applications of Micropipettes:

Chemical Laboratories: Micropipettes are used for precise handling of volatile and viscous samples.

Pharmaceutical Laboratories: They play a key role in quality control (QC) assessments of pharmaceutical products.

Molecular Biology Laboratories: Micropipettes are essential for experiments like PCR, western blotting, and other molecular biology procedures.

 

Uses of Micropipettes:

• Preparing samples and reagents with accurate volume measurements.
• Lightly mixing samples by repeatedly pressing and releasing the plunger.
• Adding specific volumes of solvents for dilution.
• Transferring small sample volumes between tubes.
• Measuring and dispensing small quantities in experiments such as ELISA, PCR, and molecular biology studies.

 

Choosing the Right Micropipette tips:

Micropipette tips are disposable attachments that come in various shapes, sizes, and materials, each designed for specific purposes. Key factors to consider include:

Volume Range: Tips are designed for specific volume capacities.

Material: Common materials include polypropylene and low-retention variants.

Sterility: Tips can be sterile or non-sterile based on application needs.

Compatibility: Ensure tips fit your specific micropipette model.

Volume Ranges and Tip Sizes

Low-Volume Tips (0.1 – 10 µL) Ideal for PCR and molecular biology where precision is critical. Narrow design for precise delivery; filtered tips can help prevent contamination.

Medium-Volume Tips (10 – 200 µL) Suitable for general lab work and enzyme assays. Versatile, offering a good balance between accuracy and volume capacity.

High-Volume Tips (200 – 1000 µL) Best for protein purification and large-volume transfers. Sturdy construction for high-volume measurements; extended-length options are available for deep containers.

 

Material Considerations:

Standard Polypropylene Tips - Cost-effective and versatile for general use.May retain small amounts of liquid, affecting accuracy for very low volumes.

Low-Retention Tips- Minimize liquid retention, enhancing precision, especially for viscous liquids. Ideal for assays where every microliter counts.

Filtered Tips: Prevent aerosols and liquids from entering the micropipette, reducing contamination risks. Essential for PCR and handling hazardous materials.

Sterile Tips: Necessary for tissue culture and microbiology to prevent contamination. Individually packaged to ensure safety.

Non-Sterile Tips: Suitable for routine lab tasks. More affordable and available in bulk.

Universal Fit Tips: Can be used with various micropipette brands for flexibility. May not provide the optimal fit, affecting accuracy.

Manufacturer-Specific Tips: Tailored for specific micropipette models, ensuring maximum accuracy. Typically more expensive but beneficial for precision-critical applications.

Extended Length Tips: Ideal for deep-well plates and hard-to-reach samples.

Gel-Loading Tips: Used for precise loading in electrophoresis.

Barrier Tips: Essential for working with volatile or hazardous substances, preventing contamination.

 

Buy Micropipette tips online at Medikabazaar:

You can easily purchase high-quality micropipette tips online at Medikabazaar. Our extensive selection includes a variety of tips designed for different volumes and applications, ensuring you find the perfect fit for your laboratory needs. Whether you require low-retention, filtered, or sterile tips, Medikabazaar offers competitive prices and reliable delivery, making it convenient to stock up on essential lab supplies. Explore our collection today and elevate your precision in liquid handling.

 

Benefits of Purchasing Micropipette tips from Medikabazaar:

 

Brand	Type	Volume Range	Price (INR)	Quality	Affordability	Best Option
Eppendorf	Standard	10 µL - 1000 µL	₹1,200 (pack of 100)	High	Moderate	Best for precision applications
Thermo Fisher	Low-Retention	0.1 µL - 10 µL	₹1,000 (pack of 50)	High	Moderate	Ideal for viscous liquids
Axygen	Filtered	10 µL - 200 µL	₹850 (pack of 100)	High	Affordable	Excellent for contamination prevention
Corning	Standard	10 µL - 1000 µL	₹800 (pack of 100)	Good	Affordable	Good balance of quality and price
Himedia	Non-Sterile	10 µL - 200 µL	₹600 (pack of 100)	Good	Very Affordable	Best for routine lab tasks
Accumax	Manufacturer-Specific	10 µL - 1000 µL	₹1,500 (pack of 100)	High	Moderate	Best for high precision work
Bio-Rad	Gel-Loading	10 µL - 200 µL	₹1,000 (pack of 100)	High	Moderate	Ideal for electrophoresis

 

Micropipette tips Price at Medikabazaar:

Our website offers an extensive selection of micropipette tips from top brands, including Accufine, Tarsons, and many others. You can compare micropipette tips prices, explore different models, and find the micropipette tips that best suit your laboratory needs.

 

Products

Accufine Gilson Micropipette Tip White Graduated

Gilson Micro Micropipette Tip

Tarsons Micro PP Autoclavable Micropipette Tip

 

FAQ’s:

 

Q1. What are micropipette tips?

Ans. Micropipette tips are disposable attachments that fit onto the end of micropipettes, designed for the precise transfer of small liquid volumes. Available in various sizes and materials, they ensure accurate measurements and prevent contamination in laboratory applications.

 

Q2. What are two things you should never do when using a micropipette?

Ans. Never use a micropipette and tips without a suitable tip attached, as this can lead to inaccurate measurements and damage to the pipette. Additionally, avoid exceeding the specified volume capacity of the micropipette, as this can cause liquid to enter the pipette body and result in contamination or malfunction.

 

Q3. What is the volume of micropipette tips?

Ans. Micropipette tips are designed to accommodate a range of volumes, typically from 0.1 µL to 1000 µL, depending on the type of tip used. Low-volume tips are ideal for small quantities, while high-volume tips handle larger liquid volumes efficiently.

 

Q4. What colour are micropipette tips?

Ans. Micropipette and tips come in various colours, often indicating the volume range they are designed for, such as yellow for low volumes (0.1–10 µL), blue for medium volumes (10–200 µL), and clear or white for high volumes (200–1000 µL). The colour coding helps users quickly identify the appropriate tips for their specific applications.

 

Q5. How many times can you use a micropipette tip?

Ans. Micropipette with tips are designed for single use to ensure accuracy and prevent contamination. Reusing tips can compromise experimental results and lead to cross-contamination between samples.