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Recombigen Uric Acid Liquid Reagents - 5x50ml (BC-505767-050)
Recombigen Uric Acid Liquid Reagents - 5x50ml (BC-505767-050): Image 1
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Recombigen Uric Acid Liquid Reagents - 5x50ml (BC-505767-050)

₹2,126

₹3,450

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Brand Recombigen

The Recombigen Uric Acid (Uricase-POD) assay is a single-reagent system designed for the quantitative determination of uric acid in serum, plasma, or urine samples. The method uses the uricase enzyme to specifically catalyze the oxidation of uric acid to allantoin, with the formation of hydrogen peroxide (H₂O₂) as a byproduct. The hydrogen peroxide is then detected through a peroxidase-catalyzed reaction that produces a color change. The intensity of the color formed is proportional to the concentration of uric acid in the sample, and it is measured spectrophotometrically.

Key Parameters:







































Parameter Description
Reagent TypeSingle Reagent
CompositionContains uricase, peroxidase (POD), and appropriate buffers for the enzymatic reaction
Pack Size5 × 50 mL
MethodUricase-POD Method <br> - Uricase oxidizes uric acid into allantoin, carbon dioxide, and hydrogen peroxide (H₂O₂) <br> - Hydrogen peroxide reacts with a chromogen in the presence of peroxidase (POD) to form a colored complex <br> - The absorbance of the colored complex is measured spectrophotometrically at a wavelength of 520–550 nm
Applications- Uric acid determination in serum, plasma, and urine <br> - Used in clinical diagnostics to assess conditions like gout, kidney stones, hyperuricemia, and renal function <br> - Commonly included in routine metabolic panels to evaluate purine metabolism and renal health
StorageStore at 2–8°C <br> Protect from light and contamination
Precautions- Ensure samples are clean and non-hemolyzed for accurate results <br> - Mix reagent well before use to ensure proper enzyme activity <br> - Measure absorbance of the reaction product at the recommended wavelength (usually 520–550 nm)

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