Athenese Leptospira IgM ELISA test kit - 96 Wells

The Athenese Leptospira IgM ELISA Test Kit (96 Wells) is a highly sensitive diagnostic assay designed for the qualitative detection of Leptospira-specific IgM antibodies in human serum or plasma. With a 96-well plate format, this ELISA kit provides a reliable and efficient solution for laboratories engaged in Leptospirosis diagnostics.

Key Benefits:

• Early and Accurate Diagnosis: High sensitivity ensures early detection of Leptospira-specific IgM antibodies, allowing for prompt diagnosis and treatment.
• Efficient Testing Workflow: The 96-well format enables simultaneous testing of multiple samples, streamlining laboratory workflows and accommodating varying testing demands.
• Qualitative Results: The test provides clear results indicating the presence or absence of antibodies, crucial for diagnosing current or recent Leptospirosis infections.
• Comprehensive Kit: All necessary components are included, simplifying the testing process and ensuring accurate, reproducible results.

The Athenese Leptospira IgM ELISA Test Kit includes:

• 1 x 96-Well Plate


• 1 x 1ml Positive Control


• 1 x 1ml Negative Control


• 1 x 11ml Sample Diluent


• 1 x 11ml Conjugate


• 1 x 20ml Wash Buffer


• 1 x 6ml Substrate A


• 1 x 6ml Substrate B


• 1 x 6ml Stop Solution


• 2 x Working Sheets


• 1 x Product Insert


• 2 x Sample T

1. Preparation: Prepare all components as per the instructions in the product insert.


2. Sample Dilution: Dilute the serum or plasma samples using the sample diluent.


3. Plate Setup: Add controls and diluted samples to the respective wells of the 96-well plate.


4. Incubation: Follow the incubation times and conditions specified in the manual.


5. Washing: Wash the wells with wash buffer as directed.


6. Detection: Add substrate solutions and allow color development.


7. Stopping the Reaction: Add stop solution to halt the reaction.


8. Result Interpretation: Read the optical density values and interpret results based on the provided cut-off.

• Handle all components in accordance with standard laboratory safety practices.


• Wear appropriate personal protective equipment (PPE) during testing.


• Dispose of all materials in compliance with local regulations for biohazard waste.