Abbott Pan Leptospira IGM ELISA test kit - Pack of 96 Wells (AF02PE10)

The Abbott Pan Leptospira IGM ELISA Test Kit (AF02PE10, Pack of 96 Wells) is a laboratory-based immunoassay designed for the qualitative detection of IgM class antibodies against Leptospira species in human serum. This in-vitro diagnostic kit aids in the clinical laboratory diagnosis of patients presenting with symptoms consistent with leptospirosis, supporting the identification of recent or acute infections.

Utilizing a standardized indirect enzyme-linked immunosorbent assay (ELISA) format, the kit employs microplates coated with specific Leptospira antigens to capture corresponding IgM antibodies from the sample. The immune complex is then detected using a horseradish peroxidase (HRP) labelled conjugate and a TMB chromogen substrate system, producing a measurable color change.

This kit is suitable for routine diagnostic use in clinical laboratories, hospitals, and epidemiological surveillance programs. It is designed to detect IgM antibodies across a broad range of Leptospira serovars.

Key Features : 

Qualitative IgM Detection: Specifically identifies IgM antibodies against Leptospira species in human serum.
Indirect ELISA Format: Employs a proven, standardized indirect enzyme-linked immunosorbent assay method.
Comprehensive Reagents: Includes antigen-coated microplate, controls, diluents, conjugate, substrate, and wash solutions.
In-Vitro Diagnostic Use: Intended for professional diagnostic testing in clinical laboratory settings.
Broad Serovar Detection: Designed to detect IgM antibodies to a wide range of Leptospira interrogans serovars.

Abbott Pan Leptospira IgM ELISA Test Kit contains:

• 96-well microtiter plate, coated with Leptospira antigen
• Enzyme-linked secondary antibody
• Wash buffer, substrate solution, and stop solution
• Sample diluent and control reagents
• Instruction manual for standardized testing

1. Collect a serum sample from the patient.
2. Add the serum sample to the antigen-coated wells.
3. Incubate the plate as per the standardized indirect ELISA protocol.
4. Wash to remove unbound substances and add enzyme-linked antibodies.
5. Apply the substrate solution to generate a colorimetric reaction.
6. Measure absorbance using an ELISA reader to interpret results.

• Use personal protective equipment (PPE) when handling specimens.
• Store the kit at 2-8°C to maintain reagent stability.
• Avoid contamination by using sterile pipettes and gloves.
• Dispose of used test components as biohazardous waste.
• Ensure proper quality control checks before performing tests.