A simple and Cost-effective protein purification system

Features : " e-Auction Description of Work : A simple and Cost-effective protein purification system " e-Auction Type : NonExclusive " e-Auction Number : NE/KIHT/20099 " Base Price : 3,80,000 " e-Auction Date / Time : 01-02-2019 15:18 " TRL : 3 " The a portable system for affinity based purification of recombinant proteins. The present invention emphasizes on the development of a less resource intensive (cost effective) protein purification system while maintaining/minimally compromising the quality of the purified protein. The system does not require a digital valve/circuit or a computer for its functioning. " Such system utilizes a combined influence of gravitational force and an aspirational pull (negative pressure) generated by a single peristaltic pump to facilitate flow of buffers between different compartments, thus making the system energy efficient. The low material cost and energy efficiency combined with ease of operation, installation, assembly and troubleshooting, strengthen the frugality of the system, thereby making it easily affordable. " In a nutshell, the present invention discloses an affinity column based protein purification system which is custom packed. As the system requires only single PP, there is a continuous flow of viscous fluids through the PC, negating the requirement of having specialized sealing of column as there is minimal risk of leakage due to negative pressure. Therefore, the system is energy efficient. Hence, the use of negative pressure and gravitational force are the key aspects of purification of proteins utilized in the system. The flow rate controllers used in the system are simple, cost effective (generally used to control flow rate of I.V. drip set) and work without any requirement of electrical energy, therefore, are energy efficient. The system involves standard silicon tubing that can sustain temperatures from 4°C to 25°C. Requirement of regular silicon tubing makes the system cost-effective and easy to assemble. Need & Demand : " Before identifying a specific protein and studying properties thereof, a protein is separated (purified) from a sample of plant or animal tissue or from a complex mixture. Therefore, extraction and purification may be vital components of almost any protein specific research effort. " While there has been an increase in the demand for the production of a variety of recombinant proteins, the methods leading to their purification, have not evolved in parallel. " The conventional methods and techniques may have certain disadvantages such as high cost, lengthy and complex purification process, immobility, and heavy weight of the installation set up, difficult to replace parts of the system, too many devices such as columns, pumps, and so on. " There exists a need for developing protein purification systems and methods thereof which may be simple, cost-effective, efficient in working, portable